Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish

Stavros Vagionitis, Tim Czopka

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

In vivo time-lapse microscopy provides important information about the kinetics of cellular events and their control by interactions with neighboring cells. Here, we describe the generation and use of transgenic zebrafish to visualize dynamics of myelinating glia using cell type-specific expression and microscopy of genetically encoded fluorescent proteins. With this method, we are able to simultaneously separate and trace up to three different colors over time.

Original languageEnglish
Title of host publicationMyelin
Pages25-35
Number of pages11
Volume1791
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Animals
  • Animals, Genetically Modified
  • Biomarkers
  • Microscopy, Fluorescence
  • Molecular Imaging/instrumentation
  • Myelin Sheath/metabolism
  • Neuroglia/metabolism
  • Time-Lapse Imaging/instrumentation
  • Zebrafish

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