Wash‐free, peptide‐based fluorogenic probes for microbial imaging

Assel Baibek, Muhammed Üçüncü, Elizabeth A. Blackburn, Mark Bradley, Annamaria Lilienkampf

Research output: Contribution to journalArticlepeer-review


Peptides with pan‐antimicrobial affinity were synthesized and decorated with environmentally sensitive fluorophores nitrobenzoxadiazole (green) and merocyanine (red). The labeling efficacies against a range of clinically relevant fungal and Gram‐negative and positive bacterial species (Candida albicans, Escherichia coli, and Staphylococcus aureus) were explored. The fluorogenic probes containing exclusively l or d‐amino acids showed rapid and efficient labeling of all microbial species, whereas probes with a mixture of l or d‐amino acids failed to label fungi or bacteria, highlighting the importance of the α‐helical peptide structure for interaction with the microbial cell membrane. Importantly, the nature of the dye allowed fluorescence detection/labeling without the need for a wash step, paving the way for direct application of the probes.
Original languageEnglish
Article numbere24167
JournalPeptide Science
Early online date7 May 2020
Publication statusE-pub ahead of print - 7 May 2020

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