Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental ST59

Zhen Xu, Raju Misra, Dorota Jamrozy, Gavin K Paterson, Ronald R Cutler, Mark A Holmes, Saheer Gharbia, Hermine V Mkrtchyan

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Staphylococcus epidermidis is a major opportunistic pathogen primarily recovered from device-associated healthcare associated infections (DA-HAIs). Although S. epidermidis and other coagulase-negative staphylococci (CoNS) are less virulent than Staphylococcus aureus, these bacteria are an important reservoir of antimicrobial resistance genes and resistance-associated mobile genetic elements that can be transferred between staphylococcal species. We report a whole genome sequence of a multidrug resistant S. epidermidis (strain G6_2) representing multilocus sequence type (ST) 59 and isolated from an environmental sampling of a hotel room in London, UK. The genome of S. epidermidis G6_2 comprises of a 2408357 bp chromosome and six plasmids, with an average G+C content of 32%. The strain displayed a multi-drug resistance phenotype which was associated with carriage of 7 antibiotic resistance genes (blaZ, mecA, msrA, mphC, fosB, aacA-aphD, tetK) as well as resistance-conferring mutations in fusA and ileS Antibiotic resistance genes were located on plasmids and chromosome. Comparative genomic analysis revealed that antibiotic resistance gene composition found in G6_2 was partly preserved across the ST59 lineage.

Original languageEnglish
Pages (from-to)2225-2230
JournalG3: Genes | Genomes | Genetics
Issue number7
Early online date30 Apr 2018
Publication statusE-pub ahead of print - 30 Apr 2018

Keywords / Materials (for Non-textual outputs)

  • Staphylococcus epidermidis
  • antibiotic resistance
  • whole genome sequence
  • comparative analysis


Dive into the research topics of 'Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental ST59'. Together they form a unique fingerprint.

Cite this