Wide-Field Multiphoton Imaging Through Scattering Media Without Correction

Adria Escobet-Montalban, Roman Spesyvtsev, Mingzhou Chen, Wardiya Afshar-Saber, Melissa R. Andrews, C Simon Herrington, Michael Mazilu, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review


Optical approaches to fluorescent, spectroscopic and morphological imaging
have made exceptional advances in the last decade. Super-resolution imaging
and wide-field multiphoton imaging are now underpinning major advances
across the biomedical sciences. Whilst the advances have been startling, the
key unmet challenge to date in all forms of optical imaging is to penetrate
deeper. A number of schemes implement aberration correction or the use of
complex photonics to address this need. In contrast, here we approach this
challenge by implementing a scheme that requires no a priori information
about the media nor its properties. Exploiting temporal focusing and singlepixel
detection in our innovative scheme we obtain wide-field two-photon images
through various turbid media including a scattering phantom and tissue
reaching a depth of up to seven scattering mean-free-path lengths. Our results
show that it competes favorably with standard point-scanning two-photon
imaging, with up to a five-fold improvement in signal-to-background ratio
while showing significantly lower photobleaching.
Original languageEnglish
JournalScience Advances
Early online date12 Oct 2018
Publication statusPublished - 12 Oct 2018

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