Widespread Protein Assembly as a Stress Response

Eukaryotic cells subjected to environmental stress respond by remodeling translation and transcription, and assembling proteins and mRNA into dynamic bodies called stress granules. How these two major components of the cells' response to heat stress are achieved remains to be understood. Here we provide a proteome-scale portrait of heat-triggered protein assembly and disassembly in budding yeast. Using a hybrid centrifugation and isotope-labeled mass spectrometry approach, we quantify formation of pelletable particles at short timescales, expanding known heat-assemblers by 6-fold.