Projects per year
Abstract / Description of output
Regenerative therapy for degenerative spine disorders requires the identification of cells that can slow down and possibly reverse degenerative processes. Here, we identify an unanticipated wound-specific notochord sheath cell subpopulation that expresses Wilms Tumor (WT) 1b following injury in zebrafish. We show that localized damage leads to Wt1b expression in sheath cells, and that wt1b+cells migrate into the wound to form a stopper-like structure, likely to maintain structural integrity. Wt1b+sheath cells are distinct in expressing cartilage and vacuolar genes, and in repressing a Wt1b-p53 transcriptional programme. At the wound, wt1b+and entpd5+ cells constitute separate, tightly-associated subpopulations. Surprisingly, wt1b expression at the site of injury is maintained even into adult stages in developing vertebrae, which form in an untypical manner via a cartilage intermediate. Given that notochord cells are retained in adult intervertebral discs, the identification of novel subpopulations may have important implications for regenerative spine disorder treatments.
Original language | English |
---|---|
Article number | e30657 |
Number of pages | 26 |
Journal | eLIFE |
Volume | 7 |
Early online date | 6 Feb 2018 |
DOIs | |
Publication status | Published - 13 Feb 2018 |
Fingerprint
Dive into the research topics of 'Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair'. Together they form a unique fingerprint.Projects
- 1 Finished
Profiles
-
Elizabeth Patton
- Deanery of Molecular, Genetic and Population Health Sciences - Personal Chair of Melanoma Genetics and Drug Discovery
- MRC Human Genetics Unit
Person: Academic: Research Active