Projects per year
Abstract
Quantum dots are semiconductor fluorescent nanocrystals that exhibit excellent characteristics compared with more commonly used organic fluorescent dyes. For many years quantum dot conjugated products have been available in multiple forms for fluorescence imaging of tissue sections under the trademark name Qdot®. They have much increased brightness, narrow emission spectrum, large Stokes shift and photostability compared with conventional organic fluorescent dyes, which together make them the fluorophores of choice for demanding requirements. Vivid Qdots are recent replacements for original Qdots, modified to improve brightness, however this has affected the fluorescence stability in commonly used conditions for immunohistochemistry. We present here our investigation of the stability of original and Vivid Qdots in solution and in immunohistochemistry, highlight the potential pitfalls and propose a protocol for stable and reliable multiplex staining with current commercially available original and Vivid Qdots.
Original language | English |
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Pages (from-to) | e0163856 |
Journal | PLoS ONE |
Volume | 11 |
Issue number | 9 |
DOIs | |
Publication status | Published - 30 Sept 2016 |
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Dive into the research topics of 'Working with Commercially Available Quantum Dots for Immunofluorescence on Tissue Sections'. Together they form a unique fingerprint.Projects
- 1 Finished
Profiles
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David Kluth
- Centre for Inflammation Research
- Edinburgh Medical School - Chair of Medical Education
Person: Academic: Research Active
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Sandrine Prost
- Edinburgh Pathology
- Deanery of Clinical Sciences - Senior Lecturer
- Centre for Inflammation Research
- Edinburgh Medical School - MBChB Year 1 Director
Person: Academic: Research Active