WT1-Associated Protein-Protein Interaction Networks

Ruthrothaselvi Bharathavikru, Alex von Kriegsheim

Research output: Chapter in Book/Report/Conference proceedingChapter


Tumor-suppressor protein Wt1 has been shown to interact with specific proteins that influence its function. These protein interactions have been identified as direct individual interactions but with the potential to exist as a part of a multiprotein complex. In order to obtain the global proteome interaction map of Wt1, an unbiased label-free endogenous immunoprecipitation was performed followed by mass spectrometry to identify protein interactions that are Wt1 centric. This chapter details the different techniques that have been used to identify and characterize Wt1-interacting proteins.

Original languageEnglish
Title of host publicationSpringer Protocols
Subtitle of host publicationThe Wilms' Tumor (WT1) Gene
Number of pages8
Publication statusPublished - 15 Jul 2016


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