Xyloglucan nonasaccharide, a naturally-occurring oligosaccharin, arises in vivo by polysaccharide breakdown

Gordon J. McDougall*, Stephen C. Fry

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The production of xyloglucan nonasaccharide (XG9), an oligosaccharin that antagonises the growthpromoting action of auxin and H+, was investigated in vivo. Cell suspension cultures of spinach (Spinacia oleracea L.) were fed L[3H]fucose and the appearance of [3H]oligosaccharides and [3H]polysaccharides in the culture medium was monitored chromatographically. Two [3H]oligosaccharides accumulated in the medium — XG9 and an acylated derivative of XG9. After a short lag (∼0.5h), soluble [3H]polymers were released into the medium at a constant rate. In contrast, [3H]XG9 accumulated in the medium at a steadily accelerating rate for ∼5-8h. The data suggest a precursor→product relationship between [3H]polysaccharide and [3H]XG9. We conclude that XG9 arises by the partial hydrolysis of pre-formed xyloglucan.

Original languageEnglish
Pages (from-to)332-336
Number of pages5
JournalJournal of Plant Physiology
Volume137
Issue number3
DOIs
Publication statusPublished - 1 Jan 1991

Keywords

  • 2,4-D
  • 2,4-dichlorophenoxyacetic acid
  • Ac
  • an O-acetylated derivative of XG9
  • anti-auxin
  • auxin
  • BAW
  • BPW
  • butan-l-of/pyridine/ water (4: 3 : 4, v/v/v)
  • butan-l-ol/acetic acid/water (12:3:5, v/v/v)
  • cell walls
  • chromatographic mobility relative to that of M7
  • EAW
  • EPW
  • ethyl acetate/ pyridine/water (8:2: 1, v/v/v)
  • ethyl acetate/acetic acid/water (10: 5 : b, v/v/v)
  • L fucose
  • M7
  • maltoheptaose
  • nonasaccharide
  • oligosaccharin
  • R,
  • Spinacia oleracea
  • TFA
  • the major repeating nonasaccharide unit of primary cell wall xyloglucan (composition Glc · Xyl · Gal · Fuc), XG9 ·
  • trifluoroacetic acid
  • XG9
  • xyloglucan

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