YAC mapping by FISH using Alu-PCR-generated probes

M. Breen; B. Arveiler; I. Murray; J. R. Gosden; D. J. Porteous

YAC mapping by FISH using Alu-PCR-generated probes

M. Breen, B. Arveiler, I. Murray, J. R. Gosden, D. J. Porteous

Deanery of Molecular, Genetic and Population Health Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1-11q13.3.

Original language	English
Pages (from-to)	726-730
Number of pages	5
Journal	Genomics
Volume	13
Issue number	3
Publication status	Published - 1992

Keywords / Materials (for Non-textual outputs)

Base Sequence Chromosome Mapping/*methods Chromosomes, Fungal Chromosomes, Human, Pair 11 DNA/genetics *DNA Probes Fluorescence Gene Library *Genome, Human Humans Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction

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@article{93c6009b7e4141848a698e9809f65001,

title = "YAC mapping by FISH using Alu-PCR-generated probes",

abstract = "Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1-11q13.3.",

keywords = "Base Sequence Chromosome Mapping/*methods Chromosomes, Fungal Chromosomes, Human, Pair 11 DNA/genetics *DNA Probes Fluorescence Gene Library *Genome, Human Humans Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction",

author = "M. Breen and B. Arveiler and I. Murray and Gosden, {J. R.} and Porteous, {D. J.}",

note = "Jul YAC mapping by FISH using Alu-PCR-generated probes Journal Article Research Support, Non-U.S. Gov't United states eng",

year = "1992",

language = "English",

volume = "13",

pages = "726--730",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press",

number = "3",

}

TY - JOUR

T1 - YAC mapping by FISH using Alu-PCR-generated probes

AU - Breen, M.

AU - Arveiler, B.

AU - Murray, I.

AU - Gosden, J. R.

AU - Porteous, D. J.

N1 - Jul YAC mapping by FISH using Alu-PCR-generated probes Journal Article Research Support, Non-U.S. Gov't United states eng

PY - 1992

Y1 - 1992

N2 - Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1-11q13.3.

AB - Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1-11q13.3.

KW - Base Sequence Chromosome Mapping/methods Chromosomes, Fungal Chromosomes, Human, Pair 11 DNA/genetics DNA Probes Fluorescence Gene Library Genome, Human Humans Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction

M3 - Article

SN - 0888-7543

VL - 13

SP - 726

EP - 730

JO - Genomics

JF - Genomics

IS - 3

ER -

YAC mapping by FISH using Alu-PCR-generated probes

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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