Yeast contains small nuclear RNAs encoded by single copy genes

Jo Ann Wise*, David Tollervey, Daniel Maloney, Harold Swerdlow, Eve J. Dunn, Christine Guthrie

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We have identified a group of RNA molecules in Saccharomyces cerevisiae that appears to be equivalent to the U class of small nuclear RNAs previously described in other eucaryotes, resembling them in size, metabolic stability, 5′ cap structure, presence of modified bases, and nuclear localization. However, the yeast snRNAs differ from their counterparts in several potentially important ways. First, they are present in very low abundance, less than 200 copies per cell, as compared to 105-106 for mammalian U1-U6. Second, there appear to be more species in yeast than in any cell type previously examined. Finally, we have cloned five yeast snRNA genes, and find that each is present in a single copy per haploid genome, whereas all previously characterized snRNAs are encoded by multiple (5 to 100) gene copies. The presence of single copy genes in yeast will greatly facilitate the genetic analysis of snRNA function.

Original languageEnglish
Pages (from-to)743-751
Number of pages9
Issue number3
Publication statusPublished - 1 Dec 1983


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