Yeast two-hybrid screens implicate DISC1 in brain development and function

J. K. Millar, S. Christie, D. J. Porteous

Research output: Contribution to journalArticlepeer-review

Abstract

DISC1 is a candidate gene for involvement in the aetiology of major psychiatric illnesses including schizophrenia. We report here the results of DISC1 yeast two-hybrid screens using human foetal and adult brain libraries. Twenty-one proteins from a variety of subcellular locations were identified, consistent with observations that DISC1 occupies multiple subcellular compartments. The cellular roles of the proteins identified implicate DISC1 in several aspects of central nervous system development and function, including gene transcription, mitochondrial function, modulation of the actin cytoskeleton, neuronal migration, glutamate transmission, and signal transduction. Intriguingly, mutations in one of the proteins identified, WKL1, have been previously suggested to underlie the aetiology of catatonic schizophrenia.
Original languageEnglish
Pages (from-to)1019-1025
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume311
Issue number4
Publication statusPublished - 2003

Keywords

  • Amino Acid Sequence Brain/*embryology/*growth & development/metabolism Humans Molecular Sequence Data Nerve Tissue Proteins/*chemistry/*physiology *Peptide Library Schizophrenia/metabolism Sequence Alignment *Sequence Analysis, Protein Structure-Activity Relationship *Two-Hybrid System Techniques Yeasts/metabolism

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