Zinc finger nuclease technology heralds a new era in mammalian transgenesis

Fabienne Le Provost, Simon Lillico, Bruno Passet, Rachel Young, Bruce Whitelaw, Jean-Luc Vilotte

Research output: Contribution to journalLiterature reviewpeer-review

Abstract / Description of output

Non-mouse mammalian transgenesis is limited by its overall inefficiency and technical hurdles. Recent years have seen the emergence of two approaches that are applicable to most mammals. The first, based on lentivirus vectors, allows efficient generation of transgenic founders, most of them expressing the transgene. The second, recently applied to produce transgenic fish and mammals, takes advantage of the design of specific 'DNA-scissors' for efficient introduction of subtle mutations in potentially any region of the genome. This review focuses on the potential of this latter technology to modify mammalian genomes without the need to apply challenging and less-efficient protocols. We highlight the complementary aims of these new approaches and the as-yet-unexplored possibilities offered by their combination.

Original languageEnglish
Pages (from-to)134-141
Number of pages8
JournalTrends in biotechnology
Issue number3
Publication statusPublished - Mar 2010


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