Edinburgh Research Explorer

Does the Middle East Respiratory Syndrome coronavirus (MERS-CoV) recombine?

Dataset

Related Edinburgh Organisations

PublisherGitHub
Date made available28 Oct 2015

Description

Very likely, yes. This repo is what I've been up to lately and is nearly finished. Most/all of the work has been done and it shows the presence of the classical recombination triad in the MERS genome: excessive homoplasies, decay of linkage disequilibrium and the presence of alternative topologies.

Done:

GARD analyses. Show that the MERS genome has well-supported alternative tree topologies and some degree of rate heterogeneity.
LDhat analyses. Permutation tests for recombination in LDhat appear quite robust to temporal sampling and indicate LD decay. Some other tests don't do so well.
Homoplasy analyses. Ancestral sequence reconstruction using ClonalFrameML indicates excessive numbers of homoplasies in the MERS genome.
Host-polymorphism association analysis. Present data do not show the presence of "human" or "camel" alleles, but are also too poorly sampled to address the question in great detail.
Homoplasy rates integrating over possible tree topologies. Shows almost identical results to ML analysis.
Marginal likelihood estimates of models including/excluding rate heterogeneity and/or alternative tree topologies. Mostly done and appears to support a model with rate heterogeneity alone, which is not surprising given the overall appearance of recombination and the cost of the number of parameters introduced by another phylogenetic tree.
Manuscript.

Data Citation

GitHub dataset identifier MERS_recombination https://github.com/evogytis/MERS_recombination

ID: 108628310