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11β-hydroxysteroid dehydrogenase-1 deficiency alters brain energy metabolism in acute systemic inflammation

Research output: Contribution to journalArticle

Original languageEnglish
JournalBrain, Behavior, and Immunity
Early online date21 Nov 2017
DOIs
Publication statusE-pub ahead of print - 21 Nov 2017

Abstract

Chronically elevated glucocorticoid levels impair cognition and are pro-inflammatory in the brain. Deficiency or inhibition of 11-hydroxysteroid dehydrogenase type-1 (11B-HSD1), which converts inactive into active glucocorticoids, protects against glucocorticoid-associated chronic stress- or age-related cognitive impairment. Here, we hypothesised that 11B-HSD1 deficiency attenuates the brain cytokine response to inflammation. Because inflammation is associated with altered energy metabolism, we also examined the effects of 11B-HSD1 deficiency upon hippocampal energy metabolism.
Inflammation was induced in 11B-HSD1 deficient (Hsd11b1Del/Del51 ) and C57BL/6 control mice by intraperitoneal injection of lipopolysaccharide (LPS). LPS reduced circulating neutrophil and monocyte numbers and increased plasma corticosterone levels equally in C57BL/6 and Hsd11b1Del/Del 54 mice, suggesting a similar peripheral inflammatory response. However, the induction of pro-inflammatory cytokine mRNAs in the hippocampus was attenuated in Hsd11b1Del/Del mice. Principal component analysis of mRNA expression revealed a distinct metabolic response to LPS in hippocampus of Hsd11b1Del/Del mice. Expression of Pfkfb3 and Ldha, key contributors to the Warburg effect, showed greater induction in Hsd11b1Del/Del mice. Consistent with increased glycolytic flux, levels of 3-phosphoglyceraldehyde and dihydroxyacetone phosphate were reduced in hippocampus of LPS injected Hsd11b1Del/Del mice. Expression of Sdha and Sdhb, encoding subunits of succinate
dehydrogenase/complex II that determines mitochondrial reserve respiratory capacity, was induced specifically in hippocampus of LPS injected Hsd11b1Del/Del mice, together with increased levels of its product, fumarate.

These data suggest 11B-HSD1 deficiency attenuates the hippocampal pro
inflammatory response to LPS, associated with increased capacity for aerobic
glycolysis and mitochondrial ATP generation. This may provide better metabolic
support and be neuroprotective during systemic inflammation or aging.

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