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3D analysis of gene expression during limb development in the Chick

Research output: Contribution to journalMeeting abstract

Original languageUndefined/Unknown
Pages (from-to)S289, abstract 18-P015
JournalMechanisms of Development
Volume126
Issue numberSupplement 1
DOIs
Publication statusPublished - 2009
Event16th International Society of Developmental Biologists Congress 2009 - Edinburgh, United Kingdom
Duration: 6 Sep 200910 Sep 2009

Abstract

Shh signalling in the polarizing region at the posterior edge of the limb bud is pivotal in controlling digit number and pattern in the developing limb. Using microarrays we have compared gene expression levels between anterior and posterior thirds of wing buds from wild-type and talpid[3] mutant chicken embryos, in which both Gli activator and repressor function fail, and identified 1070 differentially expressed genes. These were put into 16 clusters, one of which contains Hoxd13, a gene known to be involved in digit formation. Using optical projection tomography (OPT) we have performed a 3D analysis of the expression patterns of genes in the Hoxd13-like cluster. Through this analysis we aim to identify novel genes that are modulated as a consequence of Shh signaling and therefore play a role in chicken wing development. The 3D gene expression patterns were then mapped onto a digital reference limb using AMIRA software and computational analysis was used to compare the 3D spatial patterns of gene expression, which may possibly identify syn-expression groups suggesting potential functional relationships in limb development.

Event

16th International Society of Developmental Biologists Congress 2009

6/09/0910/09/09

Edinburgh, United Kingdom

Event: Conference

ID: 4129247