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A coiled-coil affinity-based system for the controlled release of biofunctionalized gold nanoparticles from alginate hydrogels

Research output: Contribution to journalArticle

  • Audrey Roth
  • Frederic Murschel
  • Pierre-Luc Latreille
  • Vincent Martinez
  • Benoit Liberelle
  • Xavier Banquy
  • Gregory De Crescenzo

Related Edinburgh Organisations

Original languageEnglish
JournalBiomacromolecules
Early online date5 Apr 2019
DOIs
Publication statusE-pub ahead of print - 5 Apr 2019

Abstract

Affinity-based systems represent a promising solution to control the delivery of therapeutics using hydrogels. Here, we report a hybrid system that is based on the peptidic E/K coiled-coil affinity pair to mediate the release of gold nanoparticles from alginate scaffolds. On the one hand, the gold nanoparticles were functionalized with Ecoil-tagged epidermal growth factor (EGF). The
bioactivity of the grafted EGF and the bioavailability of the Ecoil moiety were confirmed by EGF receptor phosphorylation assays and by capturing the functionalized nanoparticles on a Kcoilderivatized surface, respectively. On the other hand, alginate chains were modified with azidohomoalanine Kcoil (Aha-Kcoil) by azide-alkyne click chemistry. The hybrid system was formed by dispersing NPs functionalized with Ecoil-tagged EGF in alginate hydrogels containing either 0, 10 or 20% of Kcoil-modified alginate (Alg-Kcoil). With 20% of Alg-Kcoil, the release of Ecoilfunctionalized NPs was reduced by half when compared to the release of NPs without Ecoil, whereas little to no differences were noticed with either 0 or 10% of Alg-Kcoil. Differential dynamic microscopy was used to determine the diffusion coefficient of the NPs, and the results
showed a decrease in the diffusion coefficient of Ecoil-functionalized NPs, when compared to bare pegylated NPs. Altogether, our data demonstrated that the E/K coiled-coil system can control the release of NPs in a high Kcoil content alginate gel, opening diverse applications in drug delivery.

ID: 90691314