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A novel eukaryote-like CRISPR activation tool in bacteria: features and capabilities

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Original languageEnglish
Article number1900252
Number of pages10
JournalBioEssays
Volume42
Issue number6
Early online date20 Apr 2020
DOIs
Publication statusE-pub ahead of print - 20 Apr 2020

Abstract

CRISPR activation (CRISPRa) in bacteria is an attractive method for programmable gene activation. Recently, we reported a eukaryote-like, σ54-dependent CRISPRa system. It exhibits high dynamic ranges and permits flexible target site selection. Here, we present an overview of the existing strategies of CRISPRa in bacteria, and introduce the characteristics and design principles of our CRISPRa system. We discuss possible scenarios for applying the eukaryote-like CRISPRa system with corresponding suggestions for performance optimization and future functional expansion. We envision the new eukaryote-like CRISPRa system enabling novel designs in multiplexed gene regulation and promote research in the σ54-dependent gene regulatory networks among a variety of biotechnology relevant or disease-associated bacterial species.

    Research areas

  • CRISPR activation, bacteria, σ54-dependent promoters, dxCas9, synthetic biology

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