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A Role for Stargazin in Experience-Dependent Plasticity

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Original languageEnglish
Pages (from-to)1614-1625
Number of pages12
JournalCell Reports
Volume7
Issue number5
DOIs
StatePublished - 12 Jun 2014

Abstract

During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical role for the AMPAR auxiliary subunit stargazin in this plasticity. We show that stargazin is functional at the retinogeniculate synapse and that in the absence of stargazin, the refinement of the retinogeniculate synapse is specifically disrupted during the experience-dependent phase. Importantly, we found that stargazin expression and phosphorylation increased with visual deprivation and led to reduced AMPAR rectification at the retinogeniculate synapse. To test whether stargazin plays a role in homeostatic plasticity, we turned to cultured neurons and found that stargazin phosphorylation is essential for synaptic scaling. Overall, our data reveal an important role for stargazin in regulating AMPAR abundance and composition at glutamatergic synapses during homeostatic and experience-dependent plasticity.

    Research areas

  • LONG-TERM POTENTIATION, RECEPTOR REGULATORY PROTEINS, SYNAPTIC AMPA RECEPTORS, RETINOGENICULATE SYNAPSE, VISUAL-CORTEX, HOMEOSTATIC PLASTICITY, GLUTAMATE RECEPTORS, GAMMA-SUBUNIT, PHOSPHORYLATION, TRAFFICKING

ID: 19982456