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A Transcriptional switch point during hematopoietic stem and progenitor cell ontogeny

Research output: Contribution to journalArticle

  • Daisuke Sugiyama
  • Anagha Joshi
  • Kasem Kulkeaw
  • Keai Sinn Tan
  • Tomoko Yokoo-Inoue
  • Chiyo Mizuochi-Yanagi
  • Kaori Yasuda
  • Atsushi Doi
  • Tadafumi Iino
  • Masayoshi Itoh
  • Sayaka Nagao-Sato
  • Kenzaburo Tani
  • Koichi Akashi
  • Yoshihide Hayashizaki
  • Harukazu Suzuki
  • Hideya Kawaji
  • Piero Carninci
  • Alistair R R Forrest

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Original languageEnglish
Pages (from-to)314-327
JournalStem Cells and Development
Volume26
Issue number5
Early online date16 Nov 2016
DOIs
Publication statusPublished - 24 Jan 2017

Abstract

During mammalian embryogenesis, hematopoietic stem and progenitor cells (HSPCs) originate from mesoderm-derived endothelial cells in the aorta-gonad-mesonephros (AGM) region and placenta. Later, HSPCs expand in fetal liver and migrate to bone marrow shortly before birth. Understanding global transcriptional regulation governing HSPC emergence from embryonic stem/induced pluripotent stem cells is necessary to devise clinical applications, such as novel transplantation approaches. Here, to assess transcriptional dynamics during development, we performed cap analysis of gene expression (CAGE) on 10 developmental murine HSPC populations isolated from the AGM region, placenta, fetal liver and bone marrow and identified 15,681 transcripts across HSPC ontogeny. We performed microarray analysis of AGM-derived HSPCs at 9.5 and 10.5 dpc and identified 40 differentially-expressed genes, 23 confirmed as significantly changed by real-time PCR. We conclude that a transcriptional switch point occurs in HSPC ontogeny between 9.5 and 10.5 dpc in the AGM region.

    Research areas

  • hematopoietic stem and progenitor cells, ontogeny, cap analysis of gene expression

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