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A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

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    Rights statement: Copyright: © 2013 Dolt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0062054
Original languageEnglish
Article numbere62054
JournalPLoS ONE
Volume8
Issue number4
DOIs
Publication statusPublished - 2013

Abstract

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.

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