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Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo

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  • Francis Girard
  • Francis Dziva
  • Pauline van Diemen
  • Alan D. Phillips
  • Mark P. Stevens
  • Gad Frankel

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    Rights statement: Copyright © 2007, American Society for Microbiology

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http://aem.asm.org/content/73/9/3084
Original languageEnglish
Pages (from-to)3084-3090
Number of pages7
JournalApplied and Environmental Microbiology
Volume73
Issue number9
DOIs
Publication statusPublished - May 2007

Abstract

We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.

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