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Allele-specific demethylation at an imprinted mammalian promoter

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    Rights statement: © 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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http://nar.oxfordjournals.org/content/35/20/7031
Original languageEnglish
Pages (from-to)7031-7039
Number of pages9
JournalNucleic Acids Research
Volume35
Issue number20
DOIs
Publication statusPublished - Nov 2007

Abstract

A screen for imprinted genes on mouse Chromosome 7 recently identified Inpp5f_v2, a paternally expressed retrogene lying within an intron of Inpp5f. Here, we identify a novel paternally expressed variant of the Inpp5f gene (Inpp5f_v3) that shows a number of unusual features. Inpp5f_v3 initiates from a CpG-rich repeat region adjoining two B1 elements, despite previous reports that SINEs are generally excluded from imprinted promoters. Accordingly, we find that the Inpp5f_v3 promoter acquires methylation around the time of implantation, when many repeat families undergo de novo epigenetic silencing. Methylation is then lost specifically on the paternally derived allele during the latter stages of embryonic development, resulting in imprinted transcriptional activation on the demethylated allele. Methylation analyses in embryos lacking maternal methylation imprints suggest that the primary imprinting mark resides within an intronic CpG island similar to 1 kb downstream of the Inpp5f_v3 transcriptional start site. These data support the hypothesis that SINEs can influence gene expression by attracting de novo methylation during development, a property likely to explain their exclusion from other imprinted promoters.

    Research areas

  • DNA METHYLATION, CYTOSINE METHYLATION, EMBRYONIC LETHALITY, MOUSE EMBRYO, CPG ISLAND, GERM-CELLS, GENE, RETROTRANSPOSON, EXPRESSION, TRANSCRIPTION

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