Edinburgh Research Explorer

An analysis of the feasibility of short read sequencing

Research output: Contribution to journalArticle

  • N Whiteford
  • N Haslam
  • G Weber
  • A Prugel-Bennett
  • J W Essex
  • P L Roach
  • M Bradley
  • C Neylon

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

    Final published version, 132 KB, PDF document

http://nar.oxfordjournals.org/content/33/19/e171
Original languageEnglish
Article numberE171
Pages (from-to)-
Number of pages6
JournalNucleic Acids Research
Volume33
Issue number19
DOIs
Publication statusPublished - 2005

Abstract

Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20-30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80% of human chromosome 1.

    Research areas

  • HIGH-THROUGHPUT, HUMAN GENOME, DNA, ARRAYS, CHIP

Download statistics

No data available

ID: 1514571