Edinburgh Research Explorer

An expression atlas of human primary cells: inference of gene function from coexpression networks

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions

Open

Documents

  • Download as Adobe PDF

    Rights statement: © 2013 Mabbott et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Final published version, 2 MB, PDF document

http://www.biomedcentral.com/1471-2164/14/632
Original languageEnglish
Article number632
Number of pages13
JournalBMC Genomics
Volume14
Issue number1
DOIs
Publication statusPublished - 20 Sep 2013

Abstract

The specialisation of mammalian cells in time and space requires genes associated with specific pathways and functions to be co-ordinately expressed. Here we have combined a large number of publically available microarray datasets derived from human primary cells and analysed large correlation graphs of these data.
Results: Using the network analysis tool BioLayout Express(3D) we identify robust co-associations of genes expressed in a wide variety of cell lineages. We discuss the biological significance of a number of these associations, in particular the coexpression of key transcription factors with the genes that they are likely to control.

Conclusions: We consider the regulation of genes in human primary cells and specifically in the human mononuclear phagocyte system. Of particular note is the fact that these data do not support the identity of putative markers of antigen-presenting dendritic cells, nor classification of M1 and M2 activation states, a current subject of debate within immunological field. We have provided this data resource on the BioGPS web site (http://biogps.org/dataset/2429/primary-cell-atlas/) and on macrophages. com (http://www.macrophages.com/hu-cell-atlas).

Download statistics

No data available

ID: 10492167