Edinburgh Research Explorer

An immunocytochemical assay to detect human CFTR expression following gene transfer

Research output: Contribution to journalArticle

  • H. Davidson
  • A. Wilson
  • A. Horsley
  • I. A. Pringle
  • A. C. Nairn
  • C. Stearns
  • J. Gibson
  • E. Holder
  • L. Jones
  • R. Coles
  • S. G. Sumner-Jones
  • M. Wasowicz
  • M. Manvell
  • U. Griesenbach
  • S. C. Hyde
  • D. R. Gill
  • J. Davies
  • Ewfw Alton
  • A. C. Boyd

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)272-280
Number of pages9
JournalMolecular and Cellular Probes
Volume23
Issue number6
DOIs
StatePublished - Dec 2009

Abstract

Background
To assess gene therapy treatment for cystic fibrosis (CF) in clinical trials it is essential to develop robust assays that can accurately detect transgene expression in human airway epithelial cells. Our aim was to develop a reproducible immunocytochemical assay for human CFTR protein which can measure both endogenous CFTR levels and augmented CFTR expression after gene delivery.

Methods
We characterised an antibody (G449) which satisfied the criteria for use in clinical trials. We optimised our immunocytochemistry method and identified G449 dilutions at which endogenous CFTR levels were negligible in CF samples, thus enhancing detection of transgenic CFTR protein. After developing a transfection technique for brushed human nasal epithelial cells, we transfected non-CF and CF cells with a clinically relevant CpG-free plasmid encoding human CFTR.

Results
The optimised immunocytochemistry method gave improved discrimination between CF and non-CF samples. Transfection of a CFTR expression vector into primary nasal epithelial cells resulted in detectable RNA and protein expression. CFTR protein was present in 0.05–10% of non-CF cells and 0.02–0.8% of CF cells.

Conclusion
We have developed a sensitive, clinically relevant immunocytochemical assay for CFTR protein and have used it to detect transgene-expressed CFTR in transfected human primary airway epithelial cells.

    Research areas

  • CFTR antibodies, Cystic fibrosis, Gene transfer, Nasal brushing cells

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