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Book of Abstracts : CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION: CUTICLE DEPOSITION OCCURS IN THE SHELL GLAND (UTERUS) AND NOT THE VAGINA DURING THE TERMINAL PHASE OF SHELL FORMATION

Research output: Contribution to conferenceAbstract

Original languageEnglish
Publication statusPublished - 6 Sep 2017
EventEGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat -
Duration: 6 Sep 20176 Sep 2017

Conference

ConferenceEGGMEAT 2017’ XVIIth European Symposium on the Quality of Eggs and Egg Products, XXIIIth European Symposium on the Quality of Poultry Meat
Period6/09/176/09/17

Abstract

The cuticle, which forms the outermost protective layer of the egg, is produced during the terminal phase of shell formation but it is not known if it is produced in the shell gland or vagina. The aim of this study was to establish if Ovocalyxin-32 (OCX-32), a precursor protein of the eggshell cuticle is localized in the SGP or the Vagina when oviposition is endocrinologically manipulated. Immunocytochemistry for OCX- 32 was carried out on SGP and vagina tissues derived from laying hens injected with GnRH or AVT to induce premature oviposition. The % area of cells staining positive for OCX-32 was determined by analyzing 4 areas per tissue using ImageJ software. The cuticle quality and the colour of the eggs laid before and after treatment were also determined using an established method. AVT induced oviposition had a large and negative effect on cuticle and pigment deposition whereas there was little change following GnRH injection, despite both causing oviposition at the same time. The % area of ciliated surface epithelial cells staining positively for OCX-32 was higher in the AVT derived SGP tissues than for GnRH (22.7±1.7% v 16.0±1.8%; P=0.016, n=10/11). There was no specific staining for OCX-32 in the vagina from either treatment confirming that the cuticle is deposited in the shell gland pouch (uterus) and not the vagina.

ID: 50116664