Edinburgh Research Explorer

CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials

Research output: Contribution to journalArticlepeer-review

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)261-275
Number of pages15
JournalStem Cells
Volume38
Issue number2
Early online date13 Nov 2019
DOIs
Publication statusPublished - Feb 2020

Abstract

The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10- adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10- counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF-κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog-mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.

ID: 141123151