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Cellular uptake of fluorescent labelled biotin-streptavidin microspheres

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Original languageEnglish
Pages (from-to)733-739
Number of pages7
JournalJournal of fluorescence
Issue number3-4
Publication statusPublished - Jul 2008


Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.

    Research areas

  • multifunctionalised microspheres, streptavidin-biotin, flow cytometry, fluorescent imaging, intracellular delivery, PENETRATING PEPTIDES, MAMMALIAN-CELLS, DRUG-DELIVERY, DENDRIMERS, PROTEIN, AVIDIN

ID: 1481633