Edinburgh Research Explorer

CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function

Research output: Contribution to journalArticle

  • Abdelaziz Beqqali
  • Jantine Monshouwer-Kloots
  • Rui Monteiro
  • Maaike Welling
  • Jeroen Bakkers
  • Elisabeth Ehler
  • Arie Verkleij
  • Christine Mummery
  • Robert Passier

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)1141-50
Number of pages10
JournalJournal of Cell Science
Volume123
Issue numberPt 7
DOIs
Publication statusPublished - 1 Apr 2010

Abstract

In recent years, the perception of Z-disc function has changed from a passive anchor for myofilaments that allows transmission of force, to a dynamic multicomplex structure, capable of sensing and transducing extracellular signals. Here, we describe a new Z-disc protein, which we named CHAP (cytoskeletal heart-enriched actin-associated protein), expressed in differentiating heart and skeletal muscle in vitro and in vivo. Interestingly, in addition to its sarcomeric localization, CHAP was also able to translocate to the nucleus. CHAP was associated with filamentous actin in the cytoplasm and the nucleus when expressed ectopically in vitro, but in rat neonatal cardiomyocytes, CHAP disrupted the subcellular localization of alpha-actinin, another Z-disc protein. More importantly, knockdown of CHAP in zebrafish resulted in aberrant cardiac and skeletal muscle development and function. These findings suggest that CHAP is a critical component of the sarcomere with an important role in muscle development.

    Research areas

  • Active Transport, Cell Nucleus, Animals, Animals, Genetically Modified, COS Cells, Cell Nucleus, Cercopithecus aethiops, Embryo, Mammalian, Gene Knockdown Techniques, Heart, Mice, Microfilament Proteins, Muscle Development, Muscle, Skeletal, Myocytes, Cardiac, Rats, Sarcomeres, Zebrafish, Zebrafish Proteins

ID: 43362439