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Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain

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http://www.sciencedirect.com/science/article/pii/S0145305X09000901
Original languageEnglish
Pages (from-to)1018-1026
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume33
Issue number9
DOIs
Publication statusPublished - 2009

Abstract

Interleukin-7 (IL-7) is a central regulator of T cell survival and homeostasis and its expression is indicative for naive and memory T cells. We cloned chicken IL-7Ralpha (CHIL-7Ralpha) and determined its expression profile in chicken lymphocyte subpopulations. The predicted protein sequence contained 460 amino acids. The extracellular domain exhibited features typical of a type I cytokine receptor; a fibronectin type III domain and the GXWSXWS motif were conserved. ChIL-7Ralpha mRNA is highly expressed in lymphoid organs and in CD4+, CD8alpha+ and CD8beta+ cells. A monoclonal antibody was generated and expression of the protein investigated. ChIL-7Ralpha was expressed on CD4+ and CD8alpha+, but not CD8beta+, T cells, in contrast to the high mRNA expression levels in all of these cells. Upon polyclonal stimulation with ConA, IL-7Ralpha was rapidly down-regulated on T cells, suggesting that in the chicken expression of this receptor might also be correlated to the T cell activation status.

    Research areas

  • Amino Acid Sequence, Animals, Base Sequence, Chickens/immunology, Concanavalin A/pharmacology, Down-Regulation/drug effects, Down-Regulation/immunology, Lymphocyte Activation/drug effects, Molecular Sequence Data, Receptors, Interleukin-7/biosynthesis, Receptors, Interleukin-7/immunology, Sequence Alignment, T-Lymphocyte Subsets/drug effects, T-Lymphocyte Subsets/immunology

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