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Chicken anaemia virus evades host immune responses in transformed lymphocytes

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Original languageEnglish
Pages (from-to)321-327
JournalJournal of General Virology
Volume99
Issue number3
Early online date6 Feb 2018
DOIs
Publication statusE-pub ahead of print - 6 Feb 2018

Abstract

Chicken anaemia virus (CAV) is a lymphotropic virus that causes anaemia and immunosuppression in chickens. Previously, we proposed that CAV evades host antiviral responses in vivo by disrupting T-cell signalling, but the precise cellular targets and modes of action remain elusive. In this study, we examined gene expression in Marek's disease virus-transformed chicken T-cell line MSB-1 after infection with CAV using both a custom 5K immune-focused microarray and quantitative real-time PCR at 24, 48 and 72 h post-infection. The data demonstrate an intricate equilibrium between CAV and the host gene expression, displaying subtle but significant modulation of transcripts involved in the T-cell, inflammation and NF-κB signalling cascades. CAV efficiently blocked the induction of type-I interferons and interferon-stimulated genes at 72 h. The cell expression pattern implies that CAV subverts host antiviral responses and that the transformed environment of MSB-1 cells offers an opportunistic advantage for virus growth.

    Research areas

  • Chicken anaemia virus, T-lymphocytes, Immune evasion, microarray

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