Edinburgh Research Explorer

Chicken dendritic cells are susceptible to highly pathogenic avian influenza viruses which induce strong cytokine responses

Research output: Contribution to journalArticle

  • Lonneke Vervelde
  • Sylvia S. Reemers
  • Daphne A. van Haarlem
  • Jacob Post
  • Erwin Claassen
  • Johanna M. J. Rebel
  • Christine A. Jansen

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)198-206
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 2013

Abstract

Infection with highly pathogenic avian influenza (HPAI) in birds and mammals is associated with severe pathology and increased mortality. We hypothesize that in contrast to low pathogenicity avian influenza (LPAI) infection, HPAI infection of chicken dendritic cells (DC) induces a cytokine deregulation which may contribute to their highly pathogenic nature. Infection of DC with LPAI H7N1 and H5N2 resulted in viral RNA and NP expression without increase in time, in contrast to HPAI H7N1 and H5N2 mRNA expression. No increase in IFN mRNA was detected after infection with LPAI but after LPAI H5N2, and not LPAI H7N1, infection the level of bioactive IFN alpha/beta significantly increased. After HPAI H7N1 and H5N2 infection, significant increases in IL-8, IFN-alpha, IFN-gamma mRNA expression and in TLR1, 3, and 21 mRNA were observed. This enhanced activation of DC after HPAI infection may trigger deregulation of the immune response as seen during HPAI infection in chickens. (C) 2012 Elsevier Ltd. All rights reserved.

    Research areas

  • INTERFERON, Cytokine, GENE-EXPRESSION, Dendritic cell, Innate immunity, A VIRUS, ACTIVATION, NS1 PROTEIN, PATHOBIOLOGY, Pathogenicity, Influenza A virus, SWINE INFLUENZA, RECOGNITION, INFECTION, INHIBITION

ID: 7553941