Edinburgh Research Explorer

Chromatin structures of goat and sheep beta-lactoglobulin gene differ

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)649-53
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number3
DOIs
Publication statusPublished - 27 Nov 1998

Abstract

Different levels of the major milk protein beta-lactoglobulin are found in evolutionarily related ruminant species: with sheep milk containing as much as three times the concentration in goat milk. In an attempt to understand why these differences exist, we have characterised, using DNaseI as a probe of structure, the chromatin surrounding the goat beta-lactoglobulin promoter and compared it to that of the sheep homologue. The goat gene displays a mammary-specific chromatin pattern, which is reformed on expressing goat beta-lactoglobulin transgenes. This implies that this chromatin structure is sequence dependent and suggests that it plays a role in regulating beta-lactoglobulin gene expression. This pattern differs from that seen on the ovine beta-lactoglobulin gene in lactating sheep mammary chromatin. Thus, even between highly related species, the transcriptional mechanisms regulating activity of a gene can differ.

    Research areas

  • Animals, Cell Line, Chromatin, DNA Fingerprinting, Female, Goats, Lactoglobulins, Liver, Mammary Glands, Animal, Mice, Mice, Transgenic, Sheep, Species Specificity

ID: 8777437