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Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031290/
Original languageEnglish
Title of host publicationMitosis and Meiosis Part B
EditorsHelder Maiato, Melina Schuh
Place of PublicationCambridge
PublisherHUMANA PRESS INC
Chapter12
Pages237-248
Number of pages12
Volume145
Edition238454
ISBN (Print)9780128141427
DOIs
Publication statusPublished - 1 Jul 2018

Publication series

NameMethods in Cell Biology
PublisherElsevier
Volume145

Abstract

Studies using Drosophila have played pivotal roles in advancing our understanding of molecular mechanisms of mitosis throughout the past decades, due to the short generation time and advanced genetic research of this organism. Drosophila is also an excellent model to study female meiosis in oocytes. Pathways such as the acentrosomal assembly of the meiotic spindle in oocytes are conserved from fly to humans. Collecting and manipulating large Drosophila oocytes for microscopy and biochemistry is both time- and cost-efficient, offering advantages over mouse or human oocytes. Therefore, Drosophila oocytes serve as an excellent platform for molecular studies of female meiosis using a combination of genetics, microscopy and biochemistry. Here we describe key methods to observe the formation of the meiotic spindle either in fixed or in live oocytes. Moreover, biochemical methods are described to identify protein-protein interactions in vivo.

    Research areas

  • Drosophila, oocyte, spindle, microtubule, immunostaining, RNAi, fluorescent in situ hybridization, transgenesis, live-imaging, protein-protein interaction, biochemistry, immunoprecipitation

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