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Complementary Benzophenone Cross-Linking/Mass Spectrometry Photochemistry

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    Rights statement: ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. Copyright © 2017 American Chemical Society

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    Rights statement: ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.

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    Licence: Creative Commons: Attribution (CC-BY)

Original languageEnglish
Pages (from-to)5319-5324
Number of pages6
JournalAnalytical Chemistry
Volume89
Issue number10
Early online date21 Apr 2017
DOIs
Publication statusPublished - 16 May 2017

Abstract

Use of a heterobifunctional photoactivatable cross-linker, sulfo-SDA (diazirine), has yielded high-density data that facilitated structure modeling of individual proteins. We expand the photoactivatable chemistry toolbox here with a second reagent, sulfo-SBP (benzophenone). This further increases the density of photo-cross-linking to a factor of 20× over conventional cross-linking. Importantly, the two different photoactivatable groups display orthogonal directionality, enabling access to different protein regions, unreachable with a single cross-linker.

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