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Condensation of hydrodynamically stretched DNA using single molecule fluorescence imaging and optical tweezers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Original languageEnglish
Title of host publicationOPTICAL TRAPPING AND OPTICAL MICROMANIPULATION
EditorsK Dholakia, GC Spalding
Place of PublicationBELLINGHAM
PublisherSPIE
Pages225-233
Number of pages9
ISBN (Print)0-8194-5452-4
Publication statusPublished - 2004
EventConference on Optical Trapping and Optical Micromanipulation - Denver
Duration: 2 Aug 20046 Aug 2004

Conference

ConferenceConference on Optical Trapping and Optical Micromanipulation
CityDenver
Period2/08/046/08/04

Abstract

We have observed a latency time (tau) of the order 10 s in the spermidine-induced condensation of YOYO-1-labelled DNA in flow. Higher flow speeds, longer DNA and higher salt all led to an increase in tau. We propose that two effects may be relevant to these observations (1) the competition between spermidine and YOYO-1 for DNA binding sites, and (2) the bending of DNA upon spermidine binding, so that flow-induced straightening of the DNA weakens spermidine's affinity for DNA. Moreover, literature data on the mechanical properties of condensed DNA chains suggest that the hydrodynamic drag forces in our experiments should indeed significantly perturb condensation kinetics.

    Research areas

  • DNA condensation, fluorescence imaging, optical tweezers, biophysics, polymer dynamics, fluid dynamics, SEMIFLEXIBLE POLYMER-CHAIN, MULTIVALENT CATIONS, TRANSITION, BINDING

Event

Conference on Optical Trapping and Optical Micromanipulation

2/08/046/08/04

Denver

Event: Conference

ID: 1489320