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Decoding a PNA Encoded Peptide Library by PCR: The Discovery of New Cell Surface Receptor Ligands

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Original languageEnglish
Pages (from-to)1284-1289
Number of pages6
JournalChemistry and Biology
Volume18
Issue number10
DOIs
Publication statusPublished - 28 Oct 2011

Abstract

The ability to screen and identify new ligands for cell surface receptors has been a long-standing goal as it might allow targeting of pharmaceutically relevant receptors, such as integrins or G protein coupled receptors. Here, we present a method to amplify hits from a library of PNA-tagged peptides. To this end, human cells, overexpressing either integrins or the CCR6 receptor, were treated with a 10,000 member PNA-encoded peptide library. Extraction of the PNA tags from the surface of the cells was followed by a PNA-tag to DNA translation and amplification enabling decoding of the tags via microarray hybridization. This approach to ligand discovery facilitates screening for differences in surface-receptor ligands and/or receptor expression between different cell types, and opens up a practical approach to PNA-tag amplification.

    Research areas

  • SMALL-MOLECULE MICROARRAYS, COMBINATORIAL CHEMISTRY, CHEMICAL LIBRARIES, GLIOMA-CELLS, NUCLEIC-ACID, SELECTION, IDENTIFICATION, INTEGRIN, DELIVERY, DESIGN

ID: 1477217