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Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Research output: Contribution to journalArticle

  • Yue Shen
  • Yun Wang
  • Tai Chen
  • Feng Gao
  • Jianhui Gong
  • Roy Walker
  • Hongcui Zhao
  • Shihong Chen
  • Wei Luo
  • Yisha Luo
  • Carolin A Muller
  • Adrien Paul-Dubois-Taine
  • Leslie A Mitchell
  • Zhouqing Lou
  • Yanqun Fan
  • Baojin Zhou
  • Bo Wen
  • Fengji Tan
  • Yujia Wang
  • Jin Zi
  • Zexiong Xie
  • Bingzhi Li
  • Kun Yang
  • Sarah M Richardson
  • Hui Jiang
  • Conrad A. Nieduszynski
  • Romain Koszul
  • Yuan Yingjin
  • Jian Wang
  • Joel S Bader
  • Junbiao Dai
  • Jef D Boeke
  • Xun Xu
  • Huanming Yang

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Original languageEnglish
JournalScience
Volume355
Issue number6329
DOIs
StatePublished - 10 Mar 2017

Abstract

Herein we report the successful design, construction and characterization of a 770 kb synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels, including phenomics, transcriptomics, proteomics, chromosome segregation and replication analysis to provide a thorough and comprehensive analysis of a synthetic chromosome. Our “Trans-Omics” analyses reveal a modest but potentially significant pervasive up-regulation of translational machinery observed in synII is mainly caused by the deletion of 13 tRNAs. By both complementation assays and SCRaMbLE, we targeted and debuged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the HOG response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.

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