- Yue Shen
- Yun Wang
- Tai Chen
- Feng Gao
- Jianhui Gong
- Roy Walker
- Hongcui Zhao
- Shihong Chen
- Wei Luo
- Yisha Luo
- Carolin A Müller
- Adrien Paul-Dubois-Taine
- Leslie A Mitchell
- Zhouqing Lou
- Yanqun Fan
- Baojin Zhou
- Bo Wen
- Fengji Tan
- Yujia Wang
- Jin Zi
- Zexiong Xie
- Bingzhi Li
- Kun Yang
- Sarah M Richardson
- Hui Jiang
- Conrad A. Nieduszynski
- Romain Koszul
- Yuan Yingjin
- Jian Wang
- Joel S Bader
- Junbiao Dai
- Jef D Boeke
- Xun Xu
- Huanming Yang
Original language | English |
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Article number | eaaf4791 |
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Journal | Science |
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Volume | 355 |
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Issue number | 6329 |
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DOIs | |
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Publication status | Published - 10 Mar 2017 |
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Herein we report the successful design, construction and characterization of a 770 kb synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels, including phenomics, transcriptomics, proteomics, chromosome segregation and replication analysis to provide a thorough and comprehensive analysis of a synthetic chromosome. Our “Trans-Omics” analyses reveal a modest but potentially significant pervasive up-regulation of translational machinery observed in synII is mainly caused by the deletion of 13 tRNAs. By both complementation assays and SCRaMbLE, we targeted and debuged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the HOG response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.
ID: 33249810