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Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8alpha and CD28

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http://www.sciencedirect.com/science/article/pii/S0145305X08002309
Original languageEnglish
Pages (from-to)540-546
Number of pages7
JournalDevelopmental and Comparative Immunology
Volume33
Issue number4
DOIs
Publication statusPublished - Apr 2009

Abstract

The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.

    Research areas

  • Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigens, CD28, Antigens, CD4, Antigens, CD8, COS Cells, Cell Line, Cercopithecus aethiops, Chickens, Cloning, Molecular, Cross Reactions, Humans, Mice, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Turkeys

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