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Differentiation of mouse embryonic stem cells to neuronal cells using hanging droplets and retinoic acid

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Original languageEnglish
JournalBio-protocol
Volume9
Issue number21
Early online date5 Nov 2019
DOIs
Publication statusE-pub ahead of print - 5 Nov 2019

Abstract

Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.

    Research areas

  • embryonic stem cells, embryoid bodies, retinoic acid, hanging droplets, differentiation, neuronal

ID: 117346144