Edinburgh Research Explorer

Differentiation of mouse embryonic stem cells to neuronal cells using hanging droplets and retinoic acid

Research output: Contribution to journalArticle

Related Edinburgh Organisations

Open Access permissions



  • Download as Adobe PDF

    Final published version, 946 KB, PDF document

    Licence: Creative Commons: Attribution (CC-BY)

Original languageEnglish
Issue number21
Early online date5 Nov 2019
Publication statusE-pub ahead of print - 5 Nov 2019


Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.

    Research areas

  • embryonic stem cells, embryoid bodies, retinoic acid, hanging droplets, differentiation, neuronal

ID: 117346144