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Draft genome sequence of a multidrug-resistant caprine isolate of Staphylococcus cohnii subsp. urealyticus from Tanzania encoding ermB, tet(K), dfrG, fusF and fosD.

Research output: Contribution to journalArticle

  • Jeremiah Seni
  • Stephen E Mshana
  • Felician Msigwa
  • Shabani Iddi
  • Humphrey Mazigo
  • Julian Parkhill
  • Mark Holmes
  • Gavin Paterson

Related Edinburgh Organisations

Original languageEnglish
JournalJournal of global antimicrobial resistance
Early online date16 Jul 2019
DOIs
Publication statusE-pub ahead of print - 16 Jul 2019

Abstract

Objectives: Coagulase-negative staphylococci such as Staphylococcus cohnii are opportunistic pathogens of humans and animals. A multidrug-resistant isolate of Staphylococcus cohnii subsp. urealyticus, 073AN, was isolated from the nasal cavity of a healthy goat in Tanzania. This study produced and analysed a draft genome sequence of 073AN to investigate the genetic basis for antimicrobial resistance in this isolate.
Methods: 073AN was sequenced using HiSeq 2000 technology, sequencing reads assembled using Velvet and the genome annotated using Prokka.
Results: The draft genome of 073AN was 2,677,652 bp in size with a GC content of 32.5%. The isolate was resistant to several classes of antimicrobials which correlated with the presence of known antimicrobial resistance genes. Of particular note, the draft genome sequence of 073AN represents the first report of fosD in Staphylococcus cohnii and the first descriptions of fosD and fusF in Africa.
Conclusion: The draft genome sequence of S. cohnii subsp. urealyticus 073AN released here provides insight into the antimicrobial resistance determinants found in this species and in Tanzania and offers a valuable resource for further studies on staphylococcal genomics and antimicrobial resistance.

    Research areas

  • Staphylococcus cohnii, veterinary, Tanzania, antimicrobial resistance

ID: 101797383