Research output: Contribution to journal › Article
Final published version, 994 KB, PDF document
Original language | English |
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Article number | 1191 |
Journal | Nature Communications |
Volume | 8 |
Issue number | 1 |
DOIs | |
Publication status | Published - 30 Oct 2017 |
The nuclease-deactivated variant of CRISPR-Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9 or Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors.
ID: 46633044