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Enhancing the efficiency of transgene expression

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Original languageEnglish
Pages (from-to)225-32
Number of pages8
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume339
Issue number1288
DOIs
StatePublished - 27 Feb 1993

Abstract

Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.

    Research areas

  • Animals, Female, Gene Expression, Genetic Techniques, Introns, Lactoglobulins, Mammary Glands, Animal, Mice, Mice, Transgenic, Transfection

ID: 8776842