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Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

Research output: Contribution to journalArticle

  • David Bonsall
  • William F Gregory
  • Camilla L C Ip
  • Sharyne Donfield
  • James Iles
  • M Azim Ansari
  • Paolo Piazza
  • Amy Trebes
  • Anthony Brown
  • John Frater
  • Oliver G Pybus
  • Phillip Goulder
  • Paul Klenerman
  • Rory Bowden
  • Edward D Gomperts
  • Eleanor Barnes
  • Amit Kapoor
  • Colin P Sharp
  • Peter Simmonds

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    Rights statement: Copyright Emerging Infectious Diseases is published by the Centers for Disease Control and Prevention, a U.S. Government agency. Therefore, all materials published in Emerging Infectious Diseases are in the public domain and can be used without permission. Proper citation, however, is required.

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http://wwwnc.cdc.gov/eid/article/22/4/15-1812_article
Original languageEnglish
Pages (from-to)671-678
Number of pages8
JournalEmerging Infectious Diseases
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 2016

Abstract

Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.

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