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Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements

Research output: Contribution to journalArticle

  • Eva Tiecke
  • Fiona Bangs
  • Rudiger Blaschke
  • Elizabeth R Farrell
  • Gudrun Rappold
  • Cheryll Tickle

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Original languageEnglish
Pages (from-to)585-96
Number of pages12
JournalDevelopmental Biology
Volume298
Issue number2
DOIs
Publication statusPublished - 15 Oct 2006

Abstract

SHOX is a homeobox-containing gene, highly conserved among species as diverse as fish, chicken and humans. SHOX gene mutations have been shown to cause idiopathic short stature and skeletal malformations frequently observed in human patients with Turner, Leri-Weill and Langer syndromes. We cloned the chicken orthologue of SHOX, studied its expression pattern and compared this with expression of the highly related Shox2. Shox is expressed in central regions of early chick limb buds and proximal two thirds of later limbs, whereas Shox2 is expressed more posteriorly in the proximal third of the limb bud. Shox expression is inhibited distally by signals from the apical ectodermal ridge, both Fgfs and Bmps, and proximally by retinoic acid signaling. We tested Shox functions by overexpression in embryos and micromass cultures. Shox-infected chick limbs had normal proximo-distal patterning but the length of skeletal elements was consistently increased. Primary chick limb bud cell cultures infected with Shox showed an initial increase in cartilage nodules but these did not enlarge. These results fit well with the proposed role of Shox in cartilage and bone differentiation and suggest chick embryos as a useful model to study further the role of Shox in limb development.

    Research areas

  • Amino Acid Sequence, Animals, Cells, Cultured, Chick Embryo, Gene Expression Regulation, Developmental, Genes, Homeobox, Homeodomain Proteins, Limb Buds, Models, Anatomic, Molecular Sequence Data, Organ Culture Techniques, Sequence Homology, Amino Acid, Signal Transduction

ID: 13097099