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Gene Duplication and Dosage Effects During The Early Emergence of C4 Photosynthesis in The Grass Genus Alloteropsis

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  • Matheus E Bianconi
  • Luke T. Dunning
  • Jose J Moreno-Villena
  • Colin P. Osborne
  • Pascal-antoine Christin

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Original languageEnglish
Pages (from-to)1967–1980
Number of pages14
JournalJournal of Experimental Botany
Volume69
Issue number8
Early online date1 Feb 2018
DOIs
Publication statusPublished - 9 Apr 2018

Abstract

The importance of gene duplication for evolutionary diversification has been mainly discussed in terms of genetic redundancy allowing neofunctionalization. In the case of C4 photosynthesis, which evolved via the co-option of multiple enzymes to boost carbon fixation in tropical conditions, the importance of genetic redundancy has not been consistently supported by genomic studies. Here, we test for a different role of gene duplication in the early evolution of C4 photosynthesis, via dosage effects creating rapid step changes in expression levels. Using genome-wide data for accessions of the grass genus Alloteropsis that recently diversified into different photosynthetic types, we estimate gene copy numbers and demonstrate that recurrent duplications in two important families of C4 genes coincided with increases in transcript abundance along the phylogeny, in some cases via a pure dosage effect. While increased gene copy number during the initial emergence of C4 photosynthesis likely offered a rapid route to enhanced expression, we also find losses of duplicates following the acquisition of genes encoding better-suited isoforms. The dosage effect of gene duplication might therefore act as a transient process during the evolution of a C4 biochemistry, rendered obsolete by the fixation of regulatory mutations increasing expression levels.

    Research areas

  • Biochemical pathway, C4 photosynthesis, copy number variation, dosage effect, gene duplication, grasses, lowcoverage sequencing

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