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Gene regulation. Transcribed enhancers lead waves of coordinated transcription in transitioning mammalian cells

Research output: Contribution to journalArticle

  • Erik Arner
  • Carsten O Daub
  • Kristoffer Vitting-Seerup
  • Robin Andersson
  • Berit Lilje
  • Finn Drabløs
  • Andreas Lennartsson
  • Michelle Rönnerblad
  • Olga Hrydziuszko
  • Morana Vitezic
  • Tom C Freeman
  • Ahmad M N Alhendi
  • Peter Arner
  • Richard Axton
  • J Kenneth Baillie
  • Anthony Beckhouse
  • Beatrice Bodega
  • James Briggs
  • Frank Brombacher
  • Margaret Davis
  • Michael Detmar
  • Anna Ehrlund
  • Mitsuhiro Endoh
  • Afsaneh Eslami
  • Michela Fagiolini
  • Lynsey Fairbairn
  • Geoffrey J Faulkner
  • Carmelo Ferrai
  • Malcolm E Fisher
  • Lesley Forrester
  • Daniel Goldowitz
  • Reto Guler
  • Thomas Ha
  • Mitsuko Hara
  • Meenhard Herlyn
  • Tomokatsu Ikawa
  • Chieko Kai
  • Hiroshi Kawamoto
  • Levon M Khachigian
  • S Peter Klinken
  • Soichi Kojima
  • Haruhiko Koseki
  • Sarah Klein
  • Niklas Mejhert
  • Ken Miyaguchi
  • Yosuke Mizuno
  • Mitsuru Morimoto
  • Andru Tomoiu
  • Kim M Summers
  • David A Hume
  • FANTOM Consortium

Related Edinburgh Organisations

Original languageEnglish
Pages (from-to)1010-4
Number of pages5
JournalScience
Volume347
Issue number6225
Early online date12 Feb 2015
DOIs
StatePublished - 27 Feb 2015

Abstract

Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets of genes has not been previously studied en masse. Exploiting the fact that active promoters and enhancers are transcribed, we simultaneously measured their activity in 19 human and 14 mouse time courses covering a wide range of cell types and biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated the earliest responses. Binding sites for key lineage transcription factors were simultaneously overrepresented in enhancers and promoters active in each cellular system. Our data support a highly generalizable model in which enhancer transcription is the earliest event in successive waves of transcriptional change during cellular differentiation or activation.

ID: 18690018