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Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene

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  • Ki-Eun Park
  • Amy V. Kaucher
  • Anne Powell
  • Muhamad Salman Waqas
  • Shelley E.S. Sandmaier
  • Melissa J. Oatley
  • Chi-Hun Park
  • Ahmed Tibary
  • David M. Donovan
  • Le Ann Blomberg
  • Simon Lillico
  • Christopher Whitelaw
  • Alan Mileham
  • Bhanu Telugu
  • Jon M. Oatley

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    Rights statement: © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Original languageEnglish
Article number40176
JournalScientific Reports
Volume7
Early online date10 Jan 2017
DOIs
Publication statusE-pub ahead of print - 10 Jan 2017

Abstract

Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.

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