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Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian 4 Birth Cohort 1936

Research output: Contribution to journalArticle

  • Lindsay Panther
  • Loren Ornelas
  • Chunyan Liu
  • Emilda Gomez
  • Raquel Martin-Ibanez
  • Stuart J Ritchie
  • Paul Redmond
  • Lee Murphy
  • James D Cooper
  • Karen Burr
  • C. Browne
  • C.N. Svendsen
  • Sally Cowley
  • Dhruv Sareen

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Original languageEnglish
JournalStem cell research
Early online date20 May 2020
DOIs
Publication statusE-pub ahead of print - 20 May 2020

Abstract

Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non
integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

ID: 147463559